Polyclonal antibodies are basically a collection of immunoglobulins that recognize different epitopes of the same antigen molecule. After immunization, serum antibodies that can bind with the target can then be isolated through affinity purification. Not like monoclonal antibodies, polyclonal antibodies come from a variety of B cell clones, each secreting a different antibody clone. Hence, these antibodies are considered as “polyclonal” due to their heterogeneous nature.
Polyclonal antibodies serve as excellent primary antibodies in many laboratory techniques. One most important feature of polyclonal antibody is their high titer and affinity. With higher sensitivity and ability to recognize more than one site of a given antigen, polyclonal antibodies can be very superior in amplifying signals from target, getting better results in IP/ChIP. Polyclonal antibodies tolerating antigen conformational changes as well as detecting de-natured proteins. In comparison with monoclonal antibodies, polyclonal antibodies are relatively cheaper and less time-consuming which makes them a very good research tool. However, if you are looking for high selectivity or only interested in a specific antigen domain or epitope, monoclonal antibodies could be a more suitable option.
IgClue now integrates a full portfolio of polyclonal antibody products to optimize your scientific experience. We offer reliable, research-ready polyclonal antibodies (chicken, goat, rabbit, mouse, etc.) against a variety of targets.
We also provide customized solutions if you have any specific requests. Please don’t hesitate to contact us for more information.